Composite

Part:BBa_K3352007:Design

Designed by: Joyce Ting   Group: iGEM20_TAS_Taipei   (2020-10-18)


T7 + RBS Phi29 DNA Polymerase Expressing Construct


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 640
    Illegal SapI.rc site found at 1038


Design Notes

T7 RNA Polymerase has been found to be highly selective and efficient in transcribing only the T7 promoter. Resulting in almost a five-fold faster elongation rate that E. coli RNA Polymerase, T7 would be a much stronger promoter of choice.


Source

DE3 strains contain the chromosomal gene T7 RNA Polymerase which is regulated by a lac promoter.

References